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In vitro cytotoxic screening of selected Saudi medicinal ...

Sep 28, 2011· The methanolic extracts of forty species of plants traditionally used in Saudi Arabia for the treatment of a variety of diseases were tested in vitro for their potential anticancer activity on different human cancer cell lines. The cytotoxic activity of the methanolic extracts of the tested plants were determined using three human cancer cell ...

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Evaluation of the Anticancer Potential of Plants Used as ...

proliferation via the MTT assay. Using this assay, it was shown that more than half of the methanolic extracts of plants identified as having medicinal properties displayed cytotoxic or cytostatic effects against human cancer cell lines. Some of these plant extracts were more potent than others and showed selectivity for different cancer cell ...

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Cytotoxic and apoptotic effects of six herbal plants ...

Oct 31, 2011· Background Six plants from Thailand were evaluated for their cytotoxicity and apoptosis induction in human hepatocarcinoma (HepG2) as compared to normal African green monkey kidney epithelial cell lines. Methods Ethanol-water crude extracts of the six plants were tested with neutral red assay for their cytotoxicity after 24 hours of exposure to the cells. Apoptotic induction was tested in the ...

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International Journal of Pharm Tech Research

SI= IC 50 of plant extract in a normal cell line/ IC 50 of the same plant extract in cancer cell line. Results The anticancer effect of methanolic extracts of 25 plant parts against the human cancer cells HepG2, MCF-7 A549 and HCT-116 were investigated. Cultures of different cell lines were treated with extracts …

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Anticancer potential of Alternanthera sessilis extract on ...

Interestingly, A. sessilis plant parts, especially leaf extract, reduced the movement of cancer cells in a significant manner, indicating its potentiality for future use as an anti-cancer drug in colon cancer therapy. Based on these results, it can be accepted that the inhibitory effects of the leaf extracts on HT 29 cell migration may due to ...

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In vitro anticancer activity and cytotoxicity screening of ...

extract (EAE), n-butanol extract (BE), the remainder after extraction (RE) and water extract (WE) from each plant were prepared. These 60 extracts were screened for their cytotoxicity on the aforementioned cell lines using MTT assay. Results: All plant species showed certain anticancer activi-

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Phytochemical analysis and in vitro screening for ...

Antimicrobial, antioxidant and anticancer effect of the organic extract ofAcanthus ilicifolius was examined. Antimicrobial screening by disc diffusion method expressed that the plant is active againstK. pneumoniae, E. coli, and V. cholerae(08 and 01) Methanol fraction is …

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Phytochemical screening and antimicrobial activities ...

Feb 11, 2020· extract in a Petri dish using water as followed: (Weight of dish with extract – empty weight) X100 / 2 Table (2): Weight of extract obtained / weight of plant sample X100: Sample Weight of sample in gm. Weight of extract Yield % Acacia nioltica aqueous extract 40 g 6.63 g 6.63% available under aCC-BY-NC-ND 4.0 International license.

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Anti-Cancer Activity of Three Terminalia Species and ...

Table 1. Results of anticancer activity of the three methanolic plant extracts Plant Extract IC50 (ug/mL)-72 hrs incubation HT-29 HepG-2 MCF-7 MDA 3T3 (normal cell line) T. muelleri 50 40 35 n.d 130 T. bellerica 50 15 200 n.d 125 T. laxiflora n.d 140 n.d n.d n.d Identification of lowest cytotoxic dose of the plant extract for non-tumorigenic ...

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In vitro anticancer screening of South African plants ...

Oct 28, 2008· Although the extracts of the plants were randomly selected, 68% of these plant species which were hits in the screening programme are reported to be used medicinally. Based on our data, it appears that unrelated medicinal use of the source plants may serve as an initial guide to selection of plants for anticancer screening.

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Screening of antimicrobial activity of Withania somnifera ...

The pathogenic microorganisms are sub cultured 3 times at 37 0C for 24 h before the usage and 1ml of culture inoculated into nutrient broth and incubated at 37 0C for 24 h. Overnight cultures were used to test the antibacterial activity of plant extracts by using well diffusion method Table 1.

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PHYTOCHEMICAL SCREENING AND THE ANTIMICROBIAL …

2.2 Phytochemical Screening of extracts constitue. Ethanol methanol oland aqueous extracts were used for preliminary phytochemical screening using standard procedures [9]. Flavonoi. 2.3 Determination of Anti bacterial activity 2.3.1 Preparation of Culture Medium Nutrient agar medium . 1000ml of distilled water was boiled in a round

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In vitro anticancer activity and cytotoxicity screening of ...

Purpose: The purpose of this study was to evaluate the anticancer activity of 10 selected traditional Chinese medicinal plants on 4 cancer cell lines, namely MCF-7 (breast carcinoma), BALL-1 (acute lymphoblastic leukemia), Huh-7 (hepatocellular carcinoma), HeLa (cervical carcinoma) and their cytotoxicity on a normal cell line, namely MCF-10A (non-tumorigenic mammary epithelial line), about ...

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Phytochemical screening and antimicrobial activity of the ...

to anticancer. Several plants and herb species used ... Phytochemical screening of the plant extract was carried out to ... (Zaika, 1988). A 24 h old culture was used for the preparation of bacterial suspension. A suspension of organism was made in a sterile isotonic solution of sodium chloride

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Antiproliferative and apoptosis inducing potential of ...

screen the whole plant extracts of Sesuvium portulcastrum for its antiproliferative activity against various cancer cell lines. Different extracts of S. portulacastrum were prepared using ethanol, methanol, acetone, hexane, and diethyl ether as solvent. Their anticancer activity was evaluated against human breast cancer cells (MDA--231), human

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Anti-Proliferative Effects of Plant Extracts from ...

The extract from P. curatellifolia could be a potential source of lead compounds that may be used as anticancer drugs. The plant The plant extracts that showed cytotoxicity and inhibition of cell growth will be further characterized to identify the active fractions and compounds.

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Screening of in vitro antioxidant activity of methanolic ...

and root extracts were determined by using the Folin-Ciocalteu reagent according to the procedure reported by Singletion et al., (1999). About 500µl (20mg/ml) of plant sample was added to 25ml of distilled water and 1ml of Folin-Ciocalteu reagent (1:10). Then this mixture was kept at room temperature for 3 minutes, after then

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Anticancer activity of plant leaves extract collected from ...

Oct 12, 2019· The goal of this research was to explore the preliminary anticancer properties of five plants namely Calotropis procera, Moringa oleifera, Millettia pinnata, Basela alba and Euphorbia neriifolia available in Jharkhand which is used for the medicinal purpose by local tribes. In the present study, plant leaves from five species were collected, dried and extracted with solvents of increasing ...

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In-vitro anticancer activity of few plant extracts against

selected plants were prepared using soxhlet apparatus. Preliminary phytochemical studies revealed the presence of alkaloid, flavonoid, saponin, steroid, phenol, tannin, glycoside and terpenoid. In-vitro anticancer activity of selected plants extracts at various concentrations (50-250 mg/ml) was studied

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In vitro anticancer activities of Withania coagulans ...

Jun 01, 2018· Methods. The crude methanolic extracts of the root, leaf, leaf stalk, and fruit of this plant were screened for their cytotoxic activity against human (HeLa, MCF-7, RD) and rat (RG2 and INS-1) cancer cell lines at 20 μg/mL and compared to methotrexate.The IC 50 values indicated that leaf stalk and fruit extracts exert an 80% or higher cytotoxic activity against all cell lines at 24 hours.

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Phytochemicals: Extraction, Isolation, and Identification ...

Sep 22, 2017· plants Review Phytochemicals: Extraction, Isolation, and Identification of Bioactive Compounds from Plant Extracts Ammar Altemimi 1,*, Naoufal Lakhssassi 2, Azam Baharlouei 2, Dennis G. Watson 2 and David A. Lightfoot 2 1 Department of Food Science, College of Agriculture, University of Al-Basrah, Basrah 61004, Iraq 2 Department of Plant, Soil and Agricultural Systems, Plant …

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(PDF) SCREENING BIOLOGICAL ACTIVITIES OF ORTHOSIPHON ...

Screening for antibacterial activity using disc diffusion method:- The ethanolic plant extracts were tested against microorganisms namely Escherichia coli MTCC 443, Pseudomonas aeruginosa MTCC 741, Staphylococcus aureus MTCC 740, and Bacillus subtilis MTCC 10619 in order to determine the antibacterial activity.

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Evaluation of anticancer potential of Thai medicinal herb ...

May 23, 2019· The cell viability of the two CCA cell lines was assessed using SRB assay following 48 and 72 hrs of treatment with plant extracts at 62.5–1,000 μg/mL. The results showed that all CCA cell lines responded to the cytotoxic effects of the plant extract in dose- and time-dependent manners . ESD was shown to have the greatest inhibitory effect ...

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Screening of some Plants in Egypt for their Cytotoxicity ...

Seham MA Moustafa et al /Int.J. PharmTech Res.2014,6(3),pp 1074-1084. 1075 most common of which include drug resistance, toxicity, excessive hair loss, nausea and loss of appetite and the low specificity of currently available cytotoxic drugs 7, c. with the development of new technologies, a revival of collections of plants and high throughput screening for their anticancer potential is urgent ...

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Antioxidant and in vitro cytotoxic activity of extracts of ...

sources of India are likely to provide effective anti-cancer agents. These activities have been co-related to the presence of certain phytochemical substances. Herbs have a vital role in the prevention and treatment of cancer. The screening of plant extracts has been of great interest to scientists in the search for new everal diseases.

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In vitro biological screening of a critically endangered ...

Jul 23, 2018· Then, each culture was diluted, and to test the extracts against bacterial culture, 7.5 µl of crude extract was taken from 4 mg/ml DMSO stock extract in 96-well plates and serially diluted four ...

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Antitumor Activity of Aqueous Leaf Extracts of Different ...

the plants/plant extracts [12]. Potato disc tumor assay is an efficient assay to screen antitumor activity of the crude extracts of various plants, regardless of the mode of inhibitory action on tumor formation. Many scientists have used this assay to assess the antitumor potential of various plants [13-16].

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In vitro antioxidant and anticancer activity of Leea ...

Mar 01, 2017· The evaluation of the anticancer activity of plant extracts is essential for safe treatment. It enables identification of the intrinsic toxicity of the plant and the effects of acute overdose.28, 29 The MTT assay is used in screening the crude extracts as well as in the isolated compounds to assess the toxicity. It could also provide an ...

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SCREENING OF ANTIMICROBIAL, CYTOTOXIC AND …

The plant extract was screened for pesticidal activity towards Sitophilus oryzae adults. Minimum inhibitory concentration (MIC) was also studied against test organisms, using serial dilution technique to determine the antibacterial potency. In antibacterial screening, large inhibition zones were observed against the tested gram-positive

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PRELIMINARY PHYTOCHEMICAL SCREENING OF SIX …

phytochemical screening of plants is the need of the hour in order to discover and develop novel therapeutic agents with improved efficacy. Numerous research groups have also reported such studies throughout the world [4-8]. Thus, the present study deals with the screening based on phytochemical tests of six medicinal plants viz., Ficus

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In vitro evaluation of Anticancer Activity of Methnaolic ...

Oct 31, 2016· Anticancer activity using SRB assay: Sulphorhodamine B (SRB) assay was employed for screening of anticancer activity of methanolic leaves extract of Annona reticulata Linn using Human colon cancer cell line (HCT15), Human lung cancer cell line (Hop65) and Human hepatoma cell line (HEPG2). Cell lines were cultured in medium RPMI 1640 containing 10%

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Research Article Mangifera indica Extracts as Novel PKM2 ...

Feb 24, 2021· itory activity of plant extracts. Plant extracts were dissolved in DMSO to 10mg/ml, then diluted tenfold with pure water. At 25°C, 200ng/μl of test extract was incubated for 1 hour in a solution containing 100mM KCl, 50mM HEPES, 0.2mM NADH, 10mM MgCl 2, 2mM ADP, 2mM phosphoenolpyr-uvate, and 8units LDH/ml. With the help of compared

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Cytotoxic activity screening of Bangladeshi medicinal ...

Jul 12, 2013· The cytotoxic activity of 23 crude methanol extracts from 19 Bangladeshi medicinal plants was investigated against healthy mouse fibroblasts (NIH3T3), healthy monkey kidney (VERO) and four human cancer cell lines (gastric, AGS; colon, HT-29; and breast, MCF-7 and MDA--231) using MTT assay. High cytotoxicity across all cell lines tested was exhibited by Aegiceras corniculatum (fruit) and ...

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PHYTOCHEMICAL SCREENING OF ETHANOLIC EXTRACT: a ...

Phytochemical Screening of Ethanolic Extract 98 that the extract in the sample dissolves quickly in the solvent. From the five medicinal plants, the preliminary phytochemical tests of Medicinal plant extracts of different parts of five medicinal plants Parameria laevigata, Kaempferia galanga L., Curcuma domestica, Curcuma xanthorrhiza, and

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In Vitro Screening for Cytotoxic Activity of Herbal Extracts

3. Results. To demonstrate the potential biological activity of alcoholic extracts, we carried out a screening of the extracts by measuring their in vitro antiproliferative properties against several common human cancer cell lines. Some plant extracts exhibited …

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Phytochemical screening and assessment of in vitro ...

culture plate and incubated for 48 hrs to reach the confluence state of cells. Then the cells were incubated with different concentration of the plant extract (0.01, 0.1, 0.3, 0.7, 0.9, 1, 3, 5 µg/ml) for 72 hrs. Then each well was replaced with fresh media and …

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